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tobacco plant

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tobacco plant 【植物；植物學】煙草。

【植物；植物學】煙草。 “tobacco“ 中文翻譯：   n. (pl. tobaccos, tobaccoes) ...“plant“ 中文翻譯：   n. 1.植物，草木 (opp. animal); 草本 ...“songshan tobacco plant“ 中文翻譯：   松山菸廠“of tobacco“ 中文翻譯：   煙草蛙眼病“tobacco“ 中文翻譯：   n. (pl. tobaccos, tobaccoes) 【植物；植物學】煙草；煙葉；煙絲，卷煙，紙煙，嚼煙。 flue-cured tobacco 烤煙。 sun-cured tobacco 曬煙。 smoking tobacco 板絲煙。 T- Road 〔美國〕南部種植煙草的地區。 -nize 用煙葉薰；抽煙卷。 “tobacco leaf; leaf tobacco; tobacco“ 中文翻譯：   煙葉“alcohol and tobacco“ 中文翻譯：   香煙和酒精; 煙酒類“american tobacco“ 中文翻譯：   美國煙草公司“anthracnose of tobacco“ 中文翻譯：   煙草炭疽病“aromatic tobacco“ 中文翻譯：   加香的煙草; 加香煙絲; 香料配制煙; 香料煙“aztec tobacco“ 中文翻譯：   黃花煙草“baled tobacco“ 中文翻譯：   打好包的煙葉“bigleaf tobacco“ 中文翻譯：   大葉煙草“botched tobacco“ 中文翻譯：   非標準煙“brittle tobacco“ 中文翻譯：   脆性煙葉“broadleaf tobacco“ 中文翻譯：   闊葉煙, 大葉煙“bundled tobacco“ 中文翻譯：   扎成捆的煙葉“burley tobacco“ 中文翻譯：   白肋煙“cake tobacco“ 中文翻譯：   煙餅“carpet of tobacco“ 中文翻譯：   煙絲薄層“cased tobacco“ 中文翻譯：   加料煙絲“cellulose tobacco“ 中文翻譯：   纖維煙草“charred tobacco“ 中文翻譯：   燒包煙葉“chewing tobacco“ 中文翻譯：   嚼煙; 嚼用煙草“choice tobacco“ 中文翻譯：   精選級煙 草; 精選級煙草

tobacco pouch

The fact proves , and the cigarette is sold soul the depending on computer management information system of network construction , and the superiority of coming competition of tobacco plant trade is at the network , and the information technology has become the potential productive forces of enterprise . the premise to realize enterprise informationization is a business management informationization , and enterprise information development trend substance is enterprise mis ' s development trend 事實證明，卷煙銷售網絡建設的靈魂在于計算機管理信息系統，煙草行業未來競爭的優勢在網絡，信息技術已成為企業潛在的生產力。實現企業信息化的前提是企業管理信息化，企業信息化發展趨勢實質是企業mis發展趨勢。

Expression of atnced3 made the stomata of transgenic tobacco plants more sensitive promoting the somatal opening induced by light for some time and ' the stomatal closing induced by darkness . under drought stress conditions , they indicated an drastic improvement in drought tolerance than wild - type plants Atnced _ 3基因的表達對氣孔運動影響較大，明顯促進了暗誘導的氣孔關閉。在逆境條件下，轉基因煙草抗旱性顯著提高，依次是35s ， rd29a ， kst1轉基因植株，都明顯高于野生型煙草。

Cigars : tobacco products made with cigar tobacco plants which , after processing , are wrapped and rolled into anelongated form by using filling leaves as buds and wrapping with center leaves and outer leaves , or non - leaf - rolled cigars made with cigar tobacco plants as the primary ingredients and having a distinctive cigar aroma 三)雪茄?指以雪茄種菸草，經調理后以填充葉為蕊，中包葉包裹、再以外包葉卷包成長條狀之菸品，或以雪茄種菸葉為主要原料制成，菸氣中具有明顯雪茄菸香氣之非葉卷雪茄菸。

Then , we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens . after verified by antibiotic resistance , reporter gene examination , southern blot detection , and genetic segregation analysis , we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene , respectively . further , we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants 對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。

The progress present situation doing of the chemical industry of tobacco plant commerce enterprise at information be dead against is at present inquired into the problem that exists , and is analyses the position and the effect of information chemical industry work the angle from the tobacco plant commerce information construction of this text , and announces the tobacco plant business management and the relation of information system , and has put forward the basic train of thought of tobacco plant business management information system construction 本文從煙草商業信息化建設的角度，針對目前煙草商業企業在信息化工作方面的進展現狀，探討存在的問題，分析信息化工作的地位和作用，揭示煙草企業管理與信息系統的關系，提出了煙草企業管理信息系統建設的基本思路。同時，結合煙草商業企業管理信息系統建設案例，分析介紹了管理信息系統理論在企業管理活動中的實際應用效果，預示出企業信息化建設發展方向。

In transgenic tobacco plants , the transient - expression assay of the chimeric gene ( 4 x gcc - 35s min : : gus ) demonstrated that the 4 x gcc - 35smin promoter could respond to meja treatment and the gcc box is an important element in response to ja signaling . moreover , this experiment results would be meaningful to improve the crops characterization of resistance against various environment stresses or to study the regulation of gene expression in transgenic plants （ 3 ）以反向的4xgcc重復序列（ placzi 4xgcc （ 》作為placzi4xgcc的突變體， 6半乳糖苦酶活性分析的結果表明，與野生型的相比，突變的gcc元件不能與jerfi 2 3 4相互作用， p半乳糖苦酶實驗不能呈現出藍色反應；證明gccbox與jerf 2 3 4是特異性結合。

( 3 ) on the basis of the deletion analysis , three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element . transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation . loss - of - function experiment , using transient expression analysis of gus reporter genes , confirmed that gcc box act as an essential element to respond ja signaling in pdf1 . 2 promoter ( 3 )在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 ( gccbox的突變)則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。

Supervision and consultation projects completed : ( 1 ) jiangsu xindadou square ( 50 , 000m2 , two 30 - story buildings ) , worked as chief supervision engineer ( consultation ) ; ( 2 ) “ yanqiao overpass service center of xicheng highway ( the first overpass type service center on highway in china ) , worked as chief supervision engineer ( consultation ) ; ( 3 ) the ninth 5 - year - plan updating technology project of huaiyin tobacco plant , 50 , 000m2 , worked as chief ( supervision and consultation ) ; ( 4 ) the softy testing , analysis and consultations of nanjing international conference and exhibition center , site - erected structure , 75m span steel roof truss ( the largest one in china ) , worked as chief of test and consultation ; ( 5 ) “ the consultation service of the testing , analysis and update technology of the reliability of the joints of intermediate floor slabs , beams and columns of base beam of sanshanjie station of nanjing metro “ etc 完成“江蘇新大都廣場（ 5萬多平方米，二幢30層） “建設監理咨詢（總監） ； “錫澄高速堰橋跨線服務區（國內首座） “建設監理咨詢（總負責） ； “淮陰卷煙廠9 . 5技改項目（ 5萬多平方米） “建筑監理咨詢（總負責） ；主持了“南京國際會展中心75m跨鋼屋架（國內最大距）現場施工安裝結構安全性測試分析咨詢“ ； “南京地鐵三山街站中樓板及底板梁柱節點構造技術安全可靠性的測試分析與技術改進咨詢服務“等項目。

4 . after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time , incubation time and co - culture time , we set up the system of high frequency transformation of tomato cotyledons . then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation , and the resistant plants to hyg were obtained . by pcr analysis on part of the putative transformants , we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants . 5 . transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg . trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection , growth and bio - morphology analysis , hyg - resistant experiment and pcr analysis 通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，并且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。

( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1 . 2 gene promoter . these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants . the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene , and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling ( 2 )對該啟動子的5 ’端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域(有一個gccbox和一個gbox - likesequence )是其應答meja處理所必須的區域，并將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。

The result showed that oilseed crop seeds , as nest - fertilizer employed in the tobacco farmland , could strenthen the resistance of tobacco plant , improve the quality of fresh tobacco leaves , make the leaves tolerant towards maturing and roasting , increase the leave yield , improve the external appearance of the leaves , and harmonize the chemistry compositions of the leaves 結果表明，不同油料作物籽粒經處理作為窩肥配合施入煙田可增強煙株抗性，提高鮮煙葉素質，煙葉的耐成熟和耐烤性良好，從而增加煙葉產量，改善煙葉外觀質量，協調煙葉化學成分。

Expression of atnced3 driven by 35s promoter , rd29a promoter or kst1 promoter has greatly affected the growth and stomotal movement and drought tolerance in the transgenic tobacco plants . we observed the 35s : iatnced3 plants showed severe growth retarda - tion , and vd29a : atnced3 plants exhibited slight growth retardation , however we also observed the kst1 : : atnceds plants had a good growth under normal conditions 比較了35s 、 rd29a 、 kst1啟動子驅動atnced _ 3的表達對煙草生長和氣孔運動以及抗旱性的影響，結果表明35s轉基因植株明顯生長矮小，發育滯后； rd29a轉基因植株生長也比野生型慢一點； kst1轉基因陽性苗表型最好，在正常條件下生長旺盛。

The growing graphs of e . coli bl21 containing pbv221 and pbv221svhb according to the od600 values showed that the e . coli vgb + grew faster 4 the highly - efficient bivalent vectors which are named pgbif4abcvhb and pgbi4asvhbbt respectively , havfs been . su ? essful ] y constructed . the tobacco leave discs were transformed with agrobacterium tumefaciens carrying the plant expression vectors pgbif4abcvhb and pgbi4asvhbbt . 38 and 36 transgenic tobacco plants have been obtained respectively after kanamycin screening , pcr detection , southern blot analysiss showed that the foreign genes have been inserted into the tocacco plant genome . the expression of 16kd vhb protein in transgenic plants was proved by western blot 4構建了雙價基因高效植物表達載體pgbif4abcvhb和pgbi4asvhbbt 。通過農桿菌介導法，將這兩個雙價基因高效表達載體導入煙草，分別獲得了38 、 36株陽性轉基因植株。經pcr 、 southernblot分子生物學方法分析，證明vgbm ， bt基因、 bt - cpti基因已整合到煙草基因組中， westernblot證明vgbm成功地表達出16kd的vhb蛋白。

The tobacco plant commerce enterprise uses the computer and the internet work communication technology , prolonging the tip of information to the basic level network of commercial establishments , the development is applied by way of the business management information system 信息是企業生存發展的戰略資源，信息化水平已成為現代企業的重要標志。煙草商業企業采用計算機、網絡通信技術，把信息的觸點延伸到基層網點，通過商業管理信息系統開發應用。

Svde gene was also amplified from spinach by rt - pcr . the plant expression vector pcb - antisvde was constructed , in which svde gene was controlled by camv35s promoter and nos terminator . antisense transgenic tobacco plants v . ere obtained from leave explants via agrobacterium tumefaciens - medialed transformation 從菠菜中克隆了svde基因，并構建了該基因的反義抑制植物表達載體pcb - antisvde ，用根癌農桿菌介導法轉化煙草，獲得了大量的轉基因植株。

We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter , stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation 構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。

Band of c = o group around 1724 cm “ ' was suggested for the characteristic band of pha were observed on the ft - ir spectra of both of the fresh tobacco leaves and their extractions . twenty - five percent of transformed tobacco plants were detected to produce phas 提取物及新鮮轉化植株葉片用傅立葉變換紅外光譜進行定性檢測，在1724cm ~ ( - 1 )附近有特征吸收峰確定其為pha ，從而證實有25 . 00的轉基因植株已含有了目的產物。

When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin , the ratio of germinated to non - germinated seeds is 3 : 1 , which is consisted with mendel ' s first law . southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants 轉基因植株的l代種了在潮霉素培養基上的萌發數與未萌發數的比值為3 ： l ，符合單基因的盂德爾分離規律。

After 60 days of growth the leaf net weight of transgenic tobacco plants has increased by108 . 4 % . 112 . 7 % respectively compared to wild - type controls . furthermore , chlorophyll in transgenic plants has risen by 34 % . 38 % compared to wild - type controls 5與對照相比，轉基因煙草表現出葉片生長和葉綠素含量增加：在生長了60天之后，轉基因煙草植株比對照植株相同部位葉片的平均干重分別增加108 . 4